Service

Peptide Synthesis

Biotools provide customized peptide synthesis service, we can synthesize as long
as 140 amino acids. We also provide many kinds of special post-translational modification for customers to choose. The purity of the synthesized peptide may
also be adjusted according to the customers choice.

Features
● 100% sequence accuracy
● Different special modification for customers to choose
● We provide small size and big size of the synthesized peptide
● Customer can decide the purity of the peptide.

Biotools provide many types of customized peptide synthesis for the customer to choose. We can synthesize up to 140 amino acids and can choose 1~1000mg or >1000mg. According to the customer we can provide different purities such as Crude (>50%), Desalted (>60%), >70%, >75%, >75%, >80%, >85%, >90%, >95%, >98%. We also provide N-end modification, C-end modification, special amino acid modification, fluorescent dye labeling, phosphorylation..etc, for customers to choose.

※For Peptide Solubility Suggestions please click here.

Product specification

1. Synthesized peptide in lyophilized powder
(a) quantity: 1-1000mg or >1000mg
(b) purity: Desalted、Crude、>70%、>75%、>80%、>85%、>90%、>95%、>98%
(c) conformation: linear, cyclic form
(d) length: up to 140 amino acids
(e) modifications: 1. N-end modification;2. C-end modification;3. special aminoacids;4. fluorescent dye label;5. cyclic peptide;6. phosphorylated peptides
(f) special modification:
 
N,C-Terminal Modification Acetylation( N-Terminal),     HYNIC (N-Terminal)
DTPA( N-Terminal),                 Formylation ( N-Terminal),
Fatty acid ( N-Terminal),       Myristic acid ( N-Terminal)
Palmytolyl( N-Terminal),        Benzyloxycarbonylation(CBZ)
Amidation( C-Terminal),       Succinylation(Suc, N-Terminal)
Special Amino acid N-Methyl amino acid (Ala,Phe,Leu,IIe,Val,Gly,Met)
Other amino acid, Dinitrobenzoylation (Lys)
Phosphorylation (Tyr, Ser, Thr, single site)
D-Arg ,   D-Ser ,   D-Met ,  D-Lys ,   Nva ,   (D)1-Nal
D-Cys ,   D-His ,    D-Pro ,   D-Tyr ,    Nle ,    (D)2-Pal
D-Asp,    D-Thr ,   D-Val ,   DOrn ,   Hse ,    (D)4-Cl-Phe
D-Asn,    D-Trp ,   D-Phe,   Orn,       Hcy,     Lys(Me2)
D-Glu,    D-Leu,    pGlu,    Abu,      Pen,     Tyr(SO3H2)
D-Gln,    D-IIe ,     Hyp ,      Alb,       Mpa ,   Ser(octanoic acid)
Fluorescence / Dye labeling Biotin (Lys in sequence) ,                         MCA             
Biotin (N-Terminal, Y/N Ahx) ,                  p-Nitroanilide(pNA)
Biotin (Without Lys in sequence)
FITC/5-FAM (N-Terminal, Y/N Ahx) ,        AMC (C-terminal)                   
Dansyl (N-Terminal, Y/N Ahx)
Cyclic Peptide
 
Disulfide bridge 1nd ,          Disulfide bridge 3nd
Disulfide bridge 2nd ,          Amide cyclic (Side chain end)
Quenched Fluorescent Peptide EDANS/DABCYL ,                 Abz/Tyr (3-NO2)
Multiple Antigenic Peptide System Asymmetric 4 branches ,   Asymmetric 8 branches
Protein Conjugation KLH ,    BSA

2. Reports of Mass and HPLC

3. Data sheet

Working day: depends on the length and the structure of the peptide. Normally we need 4 weeks. If the difficulty of the peptide is high, then the time might be more than 4 weeks.

1. Please provide the correct amino acid sequence

2. Please check if you require special modification

Q1. The method of gene and peptide synthesis
A: Chemically or PCR method for gene synthesis. Chemically, solution form or solid form synthesis for peptide synthesis.

Q2. Can the purity be 99%?
A: You need to provide us the sequence first so we can evaluate.

Q3. What is the suggested purity for cell culture?
A: At least 90%.

Q4. What is the composition of the impurity?
A: The non-attached amino acids.

Q5. What is the suggested purity for in vivo experiment?
A: At least 90%

Q6. How do you check the purity?
A: HPLC and Mass