hsa-miR-423-5p
TOOL miRNA RT-qPCR primer/probe set is an integral part of TOOL miRNA RT-qPCR assay which is based on two-step RT-qPCR to especially target microRNAs. Each TOOLS miRNA RT-qPCR primer/probe set is packaged including two parts: one tube containing the unique designed stem-loop RT primer and the other containing a mix of the qPCR probe, forward, and reverse primers.
Features
Assay information
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Comprehensive: quantitate only mature miRNAs, not precursors
- Sensitive: sequence-specific primers
- Efficient, simple, and scalable: two-step quantitative RT-PCR assay provides high-quality results in less than three hours
Assay information
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miRBase Accession Number
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MIMAT0004748
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Chromosome Location
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chr17:30117095-30117117 (+)
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Mature miRNA Sequence:
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UGAGGGGCAGAGAGCGAGACUUU
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Species:
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Human
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Assay System:
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Probe-base qPCR assay
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Design of TOOLS miRNA RT-qPCR assay system
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| Product name | miRNA ACC |
|---|---|
| hsa-let-7a-5p | MIMAT0000099 |
| hsa-let-7b-5p | MIMAT0000063 |
| hsa-let-7c-5p | MIMAT0000064 |
| hsa-let-7d-3p | MIMAT0000433 |
| hsa-let-7d-5p | MIMAT0000065 |
| hsa-let-7f-5p | MIMAT0000067 |
| hsa-let-7g-5p | MIMAT0000414 |
| hsa-let-7i-3p | MIMAT0004585 |
| hsa-miR-1-3p | MIMAT0000416 |
| hsa-miR-9-5p | MIMAT0000441 |
| hsa-miR-9-3p | MIMAT0000442 |
| hsa-miR-10a-3p | MIMAT0004555 |
| hsa-miR-10a-5p | MIMAT0000253 |
| hsa-miR-10b-3p | MIMAT0004556 |
| hsa-miR-10b-5p | MIMAT0000254 |
| hsa-miR-15b-3p | MIMAT0004586 |
| hsa-miR-15a-5p | MIMAT0000068 |
| hsa-miR-15b-5p | MIMAT0000417 |
| hsa-miR-16-5p | MIMAT0000069 |
| hsa-miR-17-3p | MIMAT0000071 |
| hsa-miR-17-5p | MIMAT0000070 |
| hsa-miR-18a-5p | MIMAT0000072 |
| hsa-miR-18b-5p | MIMAT0001412 |
| hsa-miR-19a-3p | MIMAT0000073 |
| hsa-miR-19b-3p | MIMAT0000074 |
| hsa-miR-20a-5p | MIMAT0000075 |
| hsa-miR-21-5p | MIMAT0000076 |
| hsa-miR-22-3p | MIMAT0000077 |
| hsa-miR-22-5p | MIMAT0004495 |
| hsa-miR-23a-3p | MIMAT0000078 |
| hsa-miR-23c | MIMAT0018000 |
| hsa-miR-24-1-5p | MIMAT0000079 |
| hsa-miR-24-3p | MIMAT0000080 |
| hsa-miR-25-3p | MIMAT0000081 |
| hsa-miR-25-5p | MIMAT0004498 |
| hsa-miR-26a-5p | MIMAT0000082 |
| hsa-miR-26b-5p | MIMAT0000083 |
| hsa-miR-26a-1-3p | MIMAT0004499 |
| hsa-miR-26a-2-3p | MIMAT0004681 |
| hsa-miR-27a-3p | MIMAT0000084 |
| hsa-miR-27b-3p | MIMAT0000419 |
| hsa-miR-29a-3p | MIMAT0000086 |
| hsa-miR-29a-5p | MIMAT0004503 |
| hsa-miR-29b-2-5p | MIMAT0004515 |
| hsa-miR-29b-3p | MIMAT0000100 |
| hsa-miR-30a-3p | MIMAT0000088 |
| hsa-miR-30a-5p | MIMAT0000087 |
| hsa-miR-30b-5p | MIMAT0000420 |
| hsa-miR-30c-5p | MIMAT0000244 |
| hsa-miR-30d-5p | MIMAT0000245 |
| hsa-miR-30e-5p | MIMAT0000692 |
| hsa-miR-30e-3p | MIMAT0000693 |
| hsa-miR-31-5p | MIMAT0000089 |
| hsa-miR-32-5p | MIMAT0000090 |
| hsa-miR-33a-5p | MIMAT0000091 |
| hsa-miR-34a-5p | MIMAT0000255 |
| hsa-miR-34c-5p | MIMAT0000686 |
| hsa-miR-92a-3p | MIMAT0000092 |
| hsa-miR-92b-3p | MIMAT0003218 |
| hsa-miR-93-5p | MIMAT0000093 |
| hsa-miR-98-5p | MIMAT0000096 |
| hsa-miR-99a-5p | MIMAT0000097 |
| hsa-miR-99b-5p | MIMAT0000689 |
| hsa-miR-100-5p | MIMAT0000098 |
| hsa-miR-101-3p | MIMAT0000099 |
| hsa-miR-101-5p | MIMAT0004513 |
| hsa-miR-103a-3p | MIMAT0000101 |
| hsa-miR-106a-5p | MIMAT0000103 |
| hsa-miR-106b-3p | MIMAT0004672 |
| hsa-miR-106b-5p | MIMAT0000680 |
| hsa-miR-107 | MIMAT0000104 |
| hsa-miR-122-5p | MIMAT0000421 |
| hsa-miR-125a-5p | MIMAT0000443 |
| hsa-miR-125b-5p | MIMAT0000423 |
| hsa-miR-126-5p | MIMAT0000444 |
| hsa-miR-126-3p | MIMAT0000445 |
| hsa-miR-127-3p | MIMAT0000446 |
| hsa-miR-128-3p | MIMAT0000424 |
| hsa-miR-130b-3p | MIMAT0000691 |
| hsa-miR-130b-5p | MIMAT0004680 |
| hsa-miR-132-3p | MIMAT0000426 |
| hsa-miR-133a-3p | MIMAT0000427 |
| hsa-mir-133a-5p | MIMAT0026478 |
| hsa-miR-133b | MIMAT0000770 |
| hsa-miR-135b-5p | MIMAT0000758 |
| hsa-miR-135a-3p | MIMAT0004595 |
| hsa-miR-135a-5p | MIMAT0000428 |
| Product name | miRNA ACC |
|---|---|
| hsa-miR-139-3p | MIMAT0004552 |
| hsa-miR-140-3p | MIMAT0004597 |
| hsa-miR-141-3p | MIMAT0000432 |
| hsa-miR-142-5p | MIMAT0000433 |
| hsa-miR-142-3p | MIMAT0000434 |
| hsa-miR-143-3p | MIMAT0000435 |
| hsa-miR-144-3p | MIMAT0000436 |
| hsa-miR-144-3p | MIMAT0004600 |
| hsa-miR-145-5p | MIMAT0000437 |
| hsa-miR-146a-5p | MIMAT0000449 |
| hsa-miR-148a-3p | MIMAT0000243 |
| hsa-miR-148a-5p | MIMAT0004549 |
| hsa-miR-148b-3p | MIMAT0000759 |
| hsa-miR-149-5p | MIMAT0000450 |
| hsa-miR-150-5p | MIMAT0000451 |
| hsa-miR-151a-3p | MIMAT0000757 |
| hsa-miR-181a-3p | MIMAT0000270 |
| hsa-miR-181a-5p | MIMAT0000256 |
| hsa-miR-181b-5p | MIMAT0000257 |
| hsa-miR-182-5p | MIMAT0000259 |
| hsa-miR-183-5p | MIMAT0000261 |
| hsa-miR-184 | MIMAT0000454 |
| hsa-miR-185-5p | MIMAT0000455 |
| hsa-miR-186-5p | MIMAT0000456 |
| hsa-miR-187-3p | MIMAT0000262 |
| hsa-miR-191-5p | MIMAT0000440 |
| hsa-miR-192-5p | MIMAT0000222 |
| hsa-miR-193a-5p | MIMAT0004614 |
| hsa-miR-193b-3p | MIMAT0002819 |
| hsa-miR-193b-5p | MIMAT0004767 |
| hsa-miR-194-5p | MIMAT0000460 |
| hsa-miR-195-5p | MIMAT0000461 |
| hsa-miR-196a-5p | MIMAT0000226 |
| hsa-miR-196b-5p | MIMAT0001080 |
| hsa-miR-197-3p | MIMAT0000227 |
| hsa-miR-199a-3p | MIMAT0000232 |
| hsa-miR-199b-5p | MIMAT0000263 |
| hsa-miR-200a-3p | MIMAT0000682 |
| hsa-miR-200c-3p | MIMAT0000617 |
| hsa-miR-203a-3p | MIMAT0000264 |
| hsa-mir-204-3p | MIMAT0022693 |
| hsa-miR-204-5p | MIMAT0000265 |
| hsa-miR-205-5p | MIMAT0000266 |
| hsa-miR-208a-3p | MIMAT0000241 |
| hsa-miR-208b-3p | MIMAT0004960 |
| hsa-miR-210-3p | MIMAT0000267 |
| hsa-miR-211-5p | MIMAT0000268 |
| hsa-miR-212-3p | MIMAT0000269 |
| hsa-miR-214-3p | MIMAT0000271 |
| hsa-miR-215-5p | MIMAT0000272 |
| hsa-miR-218-5p | MIMAT0000275 |
| hsa-miR-221-3p | MIMAT0000278 |
| hsa-miR-222-3p | MIMAT0000279 |
| hsa-miR-223-3p | MIMAT0000280 |
| hsa-miR-223-5p | MIMAT0004570 |
| hsa-miR-224-5p | MIMAT0000281 |
| hsa-miR-296-3p | MIMAT0004679 |
| hsa-miR-320a-3p | MIMAT0000510 |
| hsa-miR-320d | MIMAT0006764 |
| hsa-miR-324-3p | MIMAT0000762 |
| hsa-miR-328-3p | MIMAT0000752 |
| hsa-miR-330-3p | MIMAT0000751 |
| hsa-miR-335-3p | MIMAT0004703 |
| hsa-miR-335-5p | MIMAT0000765 |
| hsa-miR-338-3p | MIMAT0000763 |
| hsa-miR-339-3p | MIMAT0004702 |
| hsa-miR-340-5p | MIMAT0004692 |
| hsa-miR-342-3p | MIMAT0000753 |
| hsa-miR-342-5p | MIMAT0004694 |
| hsa-miR-365a-3p | MIMAT0000710 |
| hsa-miR-365a-5p | MIMAT0009199 |
| hsa-miR-367-3p | MIMAT0000719 |
| hsa-miR-374b-5p | MIMAT0004955 |
| hsa-miR-375-3p | MIMAT0000728 |
| hsa-miR-378a-3p | MIMAT0000732 |
| hsa-miR-378a-5p | MIMAT0000731 |
| hsa-mir-409-3p | MIMAT0001639 |
| hsa-miR-421 | MIMAT0003339 |
| hsa-miR-423-3p | MIMAT0001340 |
| hsa-miR-423-5p | MIMAT0004748 |
| hhsa-miR-425-5p | MIMAT0003393 |
| hsa-miR-429 | MIMAT0001536 |
| hsa-miR-450a-5p | MIMAT0001545 |
| hsa-miR-451a | MIMAT0001631 |
| hsa-miR-452-5p | MIMAT0001635 |
| hsa-miR-455-3p | MIMAT0004784 |
| hsa-miR-455-5p | MIMAT0003150 |
| Product name | miRNA ACC |
|---|---|
| hsa-miR-486-3p | MIMAT0004762 |
| hsa-miR-489-3p | MIMAT0002805 |
| hsa-miR-494-3p | MIMAT0002816 |
| hsa-miR-497-5p | MIMAT0002820 |
| hsa-miR-499a-5p | MIMAT0002870 |
| hsa-miR-500a-3p | MIMAT0002871 |
| hsa-miR-501-3p | MIMAT0004774 |
| hsa-miR-502-3p | MIMAT0004775 |
| hsa-miR-506-3p | MIMAT0002878 |
| hsa-miR-508-3p | MIMAT0002880 |
| hsa-miR-509-5p | MIMAT0004779 |
| hsa-miR-514a-3p | MIMAT0002883 |
| hsa-miR-532-5p | MIMAT0002888 |
| hsa-miR-548f-5p | MIMAT0026739 |
| hsa-miR-548h-5p | MIMAT0005928 |
| hsa-miR-548ar-5p | MIMAT0022265 |
| hsa-miR-548aj-5p | MIMAT0022739 |
| hsa-miR-550a-5p | MIMAT0004800 |
| hsa-miR-574-3p | MIMAT0003239 |
| hsa-miR-576-5p | MIMAT0003241 |
| hsa-miR-582-3p | MIMAT0004797 |
| hsa-miR-584-5p | MIMAT0003249 |
| hsa-miR-615-3p | MIMAT0003283 |
| hsa-miR-619-3p | MIMAT0003288 |
| hsa-miR-624-3p | MIMAT0004807 |
| hsa-miR-625-3p | MIMAT0004808 |
| hsa-miR-625-5p | MIMAT0003294 |
| hsa-miR-627-5p | MIMAT0003296 |
| hsa-miR-629-5p | MIMAT0004810 |
| hsa-miR-633 | MIMAT0003303 |
| hsa-miR-640 | MIMAT0003310 |
| hsa-miR-644a | MIMAT0003314 |
| hsa-miR-663a | MIMAT0003326 |
| hsa-miR-664a-3p | MIMAT0005949 |
| hsa-miR-664a-5p | MIMAT0005948 |
| hsa-miR-671-5p | MIMAT0003880 |
| hsa-miR-744-5p | MIMAT0004945 |
| hsa-miR-765 | MIMAT0003945 |
| hsa-miR-877-5p | MIMAT0004949 |
| hsa-miR-888-5p | MIMAT0004916 |
| hsa-miR-933 | MIMAT0004976 |
| hsa-miR-937-3p | MIMAT0004980 |
| hsa-miR-941 | MIMAT0004984 |
| hsa-miR-1180-3p | MIMAT0005825 |
| hsa-miR-1207-5p | MIMAT0005871 |
| hsa-miR-1226-3p | MIMAT0005577 |
| hsa-miR-1227-3p | MIMAT0005580 |
| hsa-miR-1229-3p | MIMAT0005580 |
| hsa-miR-1238-3p | MIMAT0005593 |
| hsa-miR-1246 | MIMAT0005898 |
| hsa-miR-1247-5p | MIMAT0005899 |
| hsa-miR-1276 | MIMAT0005930 |
| hsa-miR-1285-3p | MIMAT0005876 |
| hsa-miR-1291 | MIMAT0005881 |
| hsa-miR-1292-5p | MIMAT0005943 |
| hsa-miR-1307-5p | MIMAT0022727 |
| hsa-miR-1322 | MIMAT0005953 |
| hsa-miR-1909-3p | MIMAT0007883 |
| hsa-miR-2110 | MIMAT0010133 |
| hsa-miR-3150a-5p | MIMAT0019206 |
| hsa-miR-3158-3p | MIMAT0015032 |
| hsa-miR-3166 | MIMAT0015040 |
| hsa-miR-3198 | MIMAT0015083 |
| hsa-miR-3615 | MIMAT0017994 |
| hsa-miR-3688-3p | MIMAT0018116 |
| hsa-miR-4260 | MIMAT0016881 |
| hsa-miR-4301 | MIMAT0016850 |
| hsa-miR-4304 | MIMAT0016854 |
| hsa-miR-4436b-5p | MIMAT0019940 |
| hsa-miR-4454 | MIMAT0018976 |
| hsa-miR-4479 | MIMAT0019011 |
| hsa-miR-4488 | MIMAT0019022 |
| hsa-miR-4732-3p | MIMAT0019856 |
| hsa-miR-4755-3p | MIMAT0019896 |
| hsa-miR-4772-5p | MIMAT0019926 |
| hsa-miR-4793-3p | MIMAT0019966 |
| hsa-miR-5684 | MIMAT0022473 |
| hsa-miR-5693 | MIMAT0022486 |
| hsa-miR-7706 | MIMAT0030021 |
| hsa-miR-7974 | MIMAT0031177 |
| hsa-miR-12136 | MIMAT0049032 |
| cel-miR-238-3p | MIMAT0000293 |
| SNORD48 | NR_002745.1 |
| SNORD44 | NR_002750.2 |
| RNU6B | NR_004394.1 |
| cel-miR-39-3p | MIMAT0000010 |
| cel-miR-54-3p | MIMAT0000025 |
TOOLS miRNA RT Kit
Cat#TTH-mi50 / TTH-mi250
TOOLS miRNA RT Kit provides the perfect first step of TOOLS miRNA RT-qPCR assay. This Kit adopts an innovative RT enzyme and buffer system, which enables the RT reaction completed within only 15 mins, and provides reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template.
Cat#TTH-mi50 / TTH-mi250
TOOLS miRNA RT Kit provides the perfect first step of TOOLS miRNA RT-qPCR assay. This Kit adopts an innovative RT enzyme and buffer system, which enables the RT reaction completed within only 15 mins, and provides reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template.
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Easy: Compatible with TOOLS miRNA RTqPCR primer/probe set, only common RT procedure required.
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Fast: RT reaction completed only within 15 mins.
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Accurate: Combination of an efficient reverse transcriptase and RNase inhibitor
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Efficient: Reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template
TOOLS Easy 2xProbe qPCR Mix
Cat#TTC-QE13
This optimized ready-to-load mix contains dNTP/dUTP mix, Mg2+, modified hot-start DNA polymerase, dUTP/UDG anticontamination system, specific ROX Reference Dye, and all components necessary to be directly used for a robust and low-template qPCR with high sensitivity, specificity, and reliability. The dUTP/UDG anticontamination system included into the mix eliminates the product contamination of qPCR reactions. TOOLS Easy 2×Probe qPCR Mix also contains ROX Reference Dye suitable for all qPCR instruments, and no adjustments are required for the dye concentration in different instruments.
Cat#TTC-QE13
This optimized ready-to-load mix contains dNTP/dUTP mix, Mg2+, modified hot-start DNA polymerase, dUTP/UDG anticontamination system, specific ROX Reference Dye, and all components necessary to be directly used for a robust and low-template qPCR with high sensitivity, specificity, and reliability. The dUTP/UDG anticontamination system included into the mix eliminates the product contamination of qPCR reactions. TOOLS Easy 2×Probe qPCR Mix also contains ROX Reference Dye suitable for all qPCR instruments, and no adjustments are required for the dye concentration in different instruments.
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Easy: All-in-one optimized master mix
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Specific: Premixed with dUTP/UDG anticontamination system
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High efficiency and stability
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ROX Reference Dye for fluorescent signal normalization and compatible with all qPCR instruments.
EasyPrep miRNA Extraction Kit
Cat#DPT-BE01
EasyPrep miRNA Extraction Kit utilizes a silica-based system to enrich and purify small RNA, including miRNA, small interfering RNA (siRNA), small nuclear RNA (snRNA), and total RNA from various sample sources (cell, animal tissue, plant tissue, serum, plasma), especially for small RNA of <200 nt. The lysis buffer in the kit is optimized to exert excellent lysis ability and isolation efficiency. High-quality isolated product without contamination of DNA and protein could be obtained within 1 hour, and directly used in Northern Blot, Dot Blot, Poly A screening, in vitro translation, RNase protection analysis, and also molecular cloning.
- Efficient: Completed within 1 hr
- Quality: silica-based system to yield high-purity small RNA product
- Flexible: Excellent lysis ability for various sample sources
Breast Cancer
- Zhao, Huanhuan, et al. "Genetic analysis and preliminary function study of miR-423 in breast cancer." Tumor Biology 36.6 (2015): 4763-4771.
- Dai, Ting, et al. "miR-423 promotes breast cancer invasion by activating NF-κB signaling." OncoTargets and therapy 13 (2020): 5467.
- Sun, Xianfu, et al. "Long non-coding RNA LINC00968 reduces cell proliferation and migration and angiogenesis in breast cancer through up-regulation of PROX1 by reducing hsa-miR-423-5p." Cell Cycle
Colorectal Cancer
- Shang, Yuanyuan, et al. "Downregulation of miR-423-5p contributes to the radioresistance in colorectal cancer cells." Frontiers in oncology 10 (2020).
- Fang, Zanxi, et al. "Plasma levels of microRNA-24, microRNA-320a, and microRNA-423-5p are potential biomarkers for colorectal carcinoma." Journal of experimental & clinical cancer research 34.1 (2015): 1-10.
- Lu, Xiaodong, and Jia Lu. "The significance of detection of serum miR-423-5p and miR-484 for diagnosis of colorectal cancer." Clinical laboratory 61.1-2 (2015): 187-190.
- Jia, Wenzhuo, et al. "MicroRNA‑423‑5p inhibits colon cancer growth by promoting caspase‑dependent apoptosis." Experimental and therapeutic medicine 16.2 (2018): 1225-1231.
- Jia, Wenrui, et al. "Association of microRNA-423 rs6505162 C> A polymorphism with susceptibility and metastasis of colorectal carcinoma." Medicine 97.6 (2018).
Parkinson Disease
- Dos Santos, Marcia Cristina T., et al. "miRNA-based signatures in cerebrospinal fluid as potential diagnostic tools for early stage Parkinson’s disease." Oncotarget 9.25 (2018): 17455.
- Martinez, Bridget, and Philip V. Peplow. "MicroRNAs in Parkinson's disease and emerging therapeutic targets." Neural Regeneration Research 12.12 (2017): 1945.