hsa-miR-195-5p
TOOL miRNA RT-qPCR primer/probe set is an integral part of TOOL miRNA RT-qPCR assay which is based on two-step RT-qPCR to especially target microRNAs. Each TOOLS miRNA RT-qPCR primer/probe set is packaged including two parts: one tube containing the unique designed stem-loop RT primer and the other containing a mix of the qPCR probe, forward, and reverse primers.
Features
Assay information
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Comprehensive: quantitate only mature miRNAs, not precursors
- Sensitive: sequence-specific primers
- Efficient, simple, and scalable: two-step quantitative RT-PCR assay provides high-quality results in less than three hours
Assay information
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miRBase Accession Number
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MIMAT0000461
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Chromosome Location
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chr17:7017667-7017687 (-)
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Mature miRNA Sequence:
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UAGCAGCACAGAAAUAUUGGC
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Species:
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Human
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Assay System:
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Probe-base qPCR assay
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Design of TOOLS miRNA RT-qPCR assay system
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| Product name | miRNA ACC |
|---|---|
| hsa-let-7a-5p | MIMAT0000099 |
| hsa-let-7b-5p | MIMAT0000063 |
| hsa-let-7c-5p | MIMAT0000064 |
| hsa-let-7d-3p | MIMAT0000433 |
| hsa-let-7d-5p | MIMAT0000065 |
| hsa-let-7f-5p | MIMAT0000067 |
| hsa-let-7g-5p | MIMAT0000414 |
| hsa-let-7i-3p | MIMAT0004585 |
| hsa-miR-1-3p | MIMAT0000416 |
| hsa-miR-9-5p | MIMAT0000441 |
| hsa-miR-9-3p | MIMAT0000442 |
| hsa-miR-10a-3p | MIMAT0004555 |
| hsa-miR-10a-5p | MIMAT0000253 |
| hsa-miR-10b-3p | MIMAT0004556 |
| hsa-miR-10b-5p | MIMAT0000254 |
| hsa-miR-15b-3p | MIMAT0004586 |
| hsa-miR-15a-5p | MIMAT0000068 |
| hsa-miR-15b-5p | MIMAT0000417 |
| hsa-miR-16-5p | MIMAT0000069 |
| hsa-miR-17-3p | MIMAT0000071 |
| hsa-miR-17-5p | MIMAT0000070 |
| hsa-miR-18a-5p | MIMAT0000072 |
| hsa-miR-18b-5p | MIMAT0001412 |
| hsa-miR-19a-3p | MIMAT0000073 |
| hsa-miR-19b-3p | MIMAT0000074 |
| hsa-miR-20a-5p | MIMAT0000075 |
| hsa-miR-21-5p | MIMAT0000076 |
| hsa-miR-22-3p | MIMAT0000077 |
| hsa-miR-22-5p | MIMAT0004495 |
| hsa-miR-23a-3p | MIMAT0000078 |
| hsa-miR-23c | MIMAT0018000 |
| hsa-miR-24-1-5p | MIMAT0000079 |
| hsa-miR-24-3p | MIMAT0000080 |
| hsa-miR-25-3p | MIMAT0000081 |
| hsa-miR-25-5p | MIMAT0004498 |
| hsa-miR-26a-5p | MIMAT0000082 |
| hsa-miR-26b-5p | MIMAT0000083 |
| hsa-miR-26a-1-3p | MIMAT0004499 |
| hsa-miR-26a-2-3p | MIMAT0004681 |
| hsa-miR-27a-3p | MIMAT0000084 |
| hsa-miR-27b-3p | MIMAT0000419 |
| hsa-miR-29a-3p | MIMAT0000086 |
| hsa-miR-29a-5p | MIMAT0004503 |
| hsa-miR-29b-2-5p | MIMAT0004515 |
| hsa-miR-29b-3p | MIMAT0000100 |
| hsa-miR-30a-3p | MIMAT0000088 |
| hsa-miR-30a-5p | MIMAT0000087 |
| hsa-miR-30b-5p | MIMAT0000420 |
| hsa-miR-30c-5p | MIMAT0000244 |
| hsa-miR-30d-5p | MIMAT0000245 |
| hsa-miR-30e-5p | MIMAT0000692 |
| hsa-miR-30e-3p | MIMAT0000693 |
| hsa-miR-31-5p | MIMAT0000089 |
| hsa-miR-32-5p | MIMAT0000090 |
| hsa-miR-33a-5p | MIMAT0000091 |
| hsa-miR-34a-5p | MIMAT0000255 |
| hsa-miR-34c-5p | MIMAT0000686 |
| hsa-miR-92a-3p | MIMAT0000092 |
| hsa-miR-92b-3p | MIMAT0003218 |
| hsa-miR-93-5p | MIMAT0000093 |
| hsa-miR-98-5p | MIMAT0000096 |
| hsa-miR-99a-5p | MIMAT0000097 |
| hsa-miR-99b-5p | MIMAT0000689 |
| hsa-miR-100-5p | MIMAT0000098 |
| hsa-miR-101-3p | MIMAT0000099 |
| hsa-miR-101-5p | MIMAT0004513 |
| hsa-miR-103a-3p | MIMAT0000101 |
| hsa-miR-106a-5p | MIMAT0000103 |
| hsa-miR-106b-3p | MIMAT0004672 |
| hsa-miR-106b-5p | MIMAT0000680 |
| hsa-miR-107 | MIMAT0000104 |
| hsa-miR-122-5p | MIMAT0000421 |
| hsa-miR-125a-5p | MIMAT0000443 |
| hsa-miR-125b-5p | MIMAT0000423 |
| hsa-miR-126-5p | MIMAT0000444 |
| hsa-miR-126-3p | MIMAT0000445 |
| hsa-miR-127-3p | MIMAT0000446 |
| hsa-miR-128-3p | MIMAT0000424 |
| hsa-miR-130b-3p | MIMAT0000691 |
| hsa-miR-130b-5p | MIMAT0004680 |
| hsa-miR-132-3p | MIMAT0000426 |
| hsa-miR-133a-3p | MIMAT0000427 |
| hsa-mir-133a-5p | MIMAT0026478 |
| hsa-miR-133b | MIMAT0000770 |
| hsa-miR-135b-5p | MIMAT0000758 |
| hsa-miR-135a-3p | MIMAT0004595 |
| hsa-miR-135a-5p | MIMAT0000428 |
| Product name | miRNA ACC |
|---|---|
| hsa-miR-139-3p | MIMAT0004552 |
| hsa-miR-140-3p | MIMAT0004597 |
| hsa-miR-141-3p | MIMAT0000432 |
| hsa-miR-142-5p | MIMAT0000433 |
| hsa-miR-142-3p | MIMAT0000434 |
| hsa-miR-143-3p | MIMAT0000435 |
| hsa-miR-144-3p | MIMAT0000436 |
| hsa-miR-144-3p | MIMAT0004600 |
| hsa-miR-145-5p | MIMAT0000437 |
| hsa-miR-146a-5p | MIMAT0000449 |
| hsa-miR-148a-3p | MIMAT0000243 |
| hsa-miR-148a-5p | MIMAT0004549 |
| hsa-miR-148b-3p | MIMAT0000759 |
| hsa-miR-149-5p | MIMAT0000450 |
| hsa-miR-150-5p | MIMAT0000451 |
| hsa-miR-151a-3p | MIMAT0000757 |
| hsa-miR-181a-3p | MIMAT0000270 |
| hsa-miR-181a-5p | MIMAT0000256 |
| hsa-miR-181b-5p | MIMAT0000257 |
| hsa-miR-182-5p | MIMAT0000259 |
| hsa-miR-183-5p | MIMAT0000261 |
| hsa-miR-184 | MIMAT0000454 |
| hsa-miR-185-5p | MIMAT0000455 |
| hsa-miR-186-5p | MIMAT0000456 |
| hsa-miR-187-3p | MIMAT0000262 |
| hsa-miR-191-5p | MIMAT0000440 |
| hsa-miR-192-5p | MIMAT0000222 |
| hsa-miR-193a-5p | MIMAT0004614 |
| hsa-miR-193b-3p | MIMAT0002819 |
| hsa-miR-193b-5p | MIMAT0004767 |
| hsa-miR-194-5p | MIMAT0000460 |
| hsa-miR-195-5p | MIMAT0000461 |
| hsa-miR-196a-5p | MIMAT0000226 |
| hsa-miR-196b-5p | MIMAT0001080 |
| hsa-miR-197-3p | MIMAT0000227 |
| hsa-miR-199a-3p | MIMAT0000232 |
| hsa-miR-199b-5p | MIMAT0000263 |
| hsa-miR-200a-3p | MIMAT0000682 |
| hsa-miR-200c-3p | MIMAT0000617 |
| hsa-miR-203a-3p | MIMAT0000264 |
| hsa-mir-204-3p | MIMAT0022693 |
| hsa-miR-204-5p | MIMAT0000265 |
| hsa-miR-205-5p | MIMAT0000266 |
| hsa-miR-208a-3p | MIMAT0000241 |
| hsa-miR-208b-3p | MIMAT0004960 |
| hsa-miR-210-3p | MIMAT0000267 |
| hsa-miR-211-5p | MIMAT0000268 |
| hsa-miR-212-3p | MIMAT0000269 |
| hsa-miR-214-3p | MIMAT0000271 |
| hsa-miR-215-5p | MIMAT0000272 |
| hsa-miR-218-5p | MIMAT0000275 |
| hsa-miR-221-3p | MIMAT0000278 |
| hsa-miR-222-3p | MIMAT0000279 |
| hsa-miR-223-3p | MIMAT0000280 |
| hsa-miR-223-5p | MIMAT0004570 |
| hsa-miR-224-5p | MIMAT0000281 |
| hsa-miR-296-3p | MIMAT0004679 |
| hsa-miR-320a-3p | MIMAT0000510 |
| hsa-miR-320d | MIMAT0006764 |
| hsa-miR-324-3p | MIMAT0000762 |
| hsa-miR-328-3p | MIMAT0000752 |
| hsa-miR-330-3p | MIMAT0000751 |
| hsa-miR-335-3p | MIMAT0004703 |
| hsa-miR-335-5p | MIMAT0000765 |
| hsa-miR-338-3p | MIMAT0000763 |
| hsa-miR-339-3p | MIMAT0004702 |
| hsa-miR-340-5p | MIMAT0004692 |
| hsa-miR-342-3p | MIMAT0000753 |
| hsa-miR-342-5p | MIMAT0004694 |
| hsa-miR-365a-3p | MIMAT0000710 |
| hsa-miR-365a-5p | MIMAT0009199 |
| hsa-miR-367-3p | MIMAT0000719 |
| hsa-miR-374b-5p | MIMAT0004955 |
| hsa-miR-375-3p | MIMAT0000728 |
| hsa-miR-378a-3p | MIMAT0000732 |
| hsa-miR-378a-5p | MIMAT0000731 |
| hsa-mir-409-3p | MIMAT0001639 |
| hsa-miR-421 | MIMAT0003339 |
| hsa-miR-423-3p | MIMAT0001340 |
| hsa-miR-423-5p | MIMAT0004748 |
| hhsa-miR-425-5p | MIMAT0003393 |
| hsa-miR-429 | MIMAT0001536 |
| hsa-miR-450a-5p | MIMAT0001545 |
| hsa-miR-451a | MIMAT0001631 |
| hsa-miR-452-5p | MIMAT0001635 |
| hsa-miR-455-3p | MIMAT0004784 |
| hsa-miR-455-5p | MIMAT0003150 |
| Product name | miRNA ACC |
|---|---|
| hsa-miR-486-3p | MIMAT0004762 |
| hsa-miR-489-3p | MIMAT0002805 |
| hsa-miR-494-3p | MIMAT0002816 |
| hsa-miR-497-5p | MIMAT0002820 |
| hsa-miR-499a-5p | MIMAT0002870 |
| hsa-miR-500a-3p | MIMAT0002871 |
| hsa-miR-501-3p | MIMAT0004774 |
| hsa-miR-502-3p | MIMAT0004775 |
| hsa-miR-506-3p | MIMAT0002878 |
| hsa-miR-508-3p | MIMAT0002880 |
| hsa-miR-509-5p | MIMAT0004779 |
| hsa-miR-514a-3p | MIMAT0002883 |
| hsa-miR-532-5p | MIMAT0002888 |
| hsa-miR-548f-5p | MIMAT0026739 |
| hsa-miR-548h-5p | MIMAT0005928 |
| hsa-miR-548ar-5p | MIMAT0022265 |
| hsa-miR-548aj-5p | MIMAT0022739 |
| hsa-miR-550a-5p | MIMAT0004800 |
| hsa-miR-574-3p | MIMAT0003239 |
| hsa-miR-576-5p | MIMAT0003241 |
| hsa-miR-582-3p | MIMAT0004797 |
| hsa-miR-584-5p | MIMAT0003249 |
| hsa-miR-615-3p | MIMAT0003283 |
| hsa-miR-619-3p | MIMAT0003288 |
| hsa-miR-624-3p | MIMAT0004807 |
| hsa-miR-625-3p | MIMAT0004808 |
| hsa-miR-625-5p | MIMAT0003294 |
| hsa-miR-627-5p | MIMAT0003296 |
| hsa-miR-629-5p | MIMAT0004810 |
| hsa-miR-633 | MIMAT0003303 |
| hsa-miR-640 | MIMAT0003310 |
| hsa-miR-644a | MIMAT0003314 |
| hsa-miR-663a | MIMAT0003326 |
| hsa-miR-664a-3p | MIMAT0005949 |
| hsa-miR-664a-5p | MIMAT0005948 |
| hsa-miR-671-5p | MIMAT0003880 |
| hsa-miR-744-5p | MIMAT0004945 |
| hsa-miR-765 | MIMAT0003945 |
| hsa-miR-877-5p | MIMAT0004949 |
| hsa-miR-888-5p | MIMAT0004916 |
| hsa-miR-933 | MIMAT0004976 |
| hsa-miR-937-3p | MIMAT0004980 |
| hsa-miR-941 | MIMAT0004984 |
| hsa-miR-1180-3p | MIMAT0005825 |
| hsa-miR-1207-5p | MIMAT0005871 |
| hsa-miR-1226-3p | MIMAT0005577 |
| hsa-miR-1227-3p | MIMAT0005580 |
| hsa-miR-1229-3p | MIMAT0005580 |
| hsa-miR-1238-3p | MIMAT0005593 |
| hsa-miR-1246 | MIMAT0005898 |
| hsa-miR-1247-5p | MIMAT0005899 |
| hsa-miR-1276 | MIMAT0005930 |
| hsa-miR-1285-3p | MIMAT0005876 |
| hsa-miR-1291 | MIMAT0005881 |
| hsa-miR-1292-5p | MIMAT0005943 |
| hsa-miR-1307-5p | MIMAT0022727 |
| hsa-miR-1322 | MIMAT0005953 |
| hsa-miR-1909-3p | MIMAT0007883 |
| hsa-miR-2110 | MIMAT0010133 |
| hsa-miR-3150a-5p | MIMAT0019206 |
| hsa-miR-3158-3p | MIMAT0015032 |
| hsa-miR-3166 | MIMAT0015040 |
| hsa-miR-3198 | MIMAT0015083 |
| hsa-miR-3615 | MIMAT0017994 |
| hsa-miR-3688-3p | MIMAT0018116 |
| hsa-miR-4260 | MIMAT0016881 |
| hsa-miR-4301 | MIMAT0016850 |
| hsa-miR-4304 | MIMAT0016854 |
| hsa-miR-4436b-5p | MIMAT0019940 |
| hsa-miR-4454 | MIMAT0018976 |
| hsa-miR-4479 | MIMAT0019011 |
| hsa-miR-4488 | MIMAT0019022 |
| hsa-miR-4732-3p | MIMAT0019856 |
| hsa-miR-4755-3p | MIMAT0019896 |
| hsa-miR-4772-5p | MIMAT0019926 |
| hsa-miR-4793-3p | MIMAT0019966 |
| hsa-miR-5684 | MIMAT0022473 |
| hsa-miR-5693 | MIMAT0022486 |
| hsa-miR-7706 | MIMAT0030021 |
| hsa-miR-7974 | MIMAT0031177 |
| hsa-miR-12136 | MIMAT0049032 |
| cel-miR-238-3p | MIMAT0000293 |
| SNORD48 | NR_002745.1 |
| SNORD44 | NR_002750.2 |
| RNU6B | NR_004394.1 |
| cel-miR-39-3p | MIMAT0000010 |
| cel-miR-54-3p | MIMAT0000025 |
TOOLS miRNA RT Kit
Cat#TTH-mi50 / TTH-mi250
TOOLS miRNA RT Kit provides the perfect first step of TOOLS miRNA RT-qPCR assay. This Kit adopts an innovative RT enzyme and buffer system, which enables the RT reaction completed within only 15 mins, and provides reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template.
Cat#TTH-mi50 / TTH-mi250
TOOLS miRNA RT Kit provides the perfect first step of TOOLS miRNA RT-qPCR assay. This Kit adopts an innovative RT enzyme and buffer system, which enables the RT reaction completed within only 15 mins, and provides reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template.
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Easy: Compatible with TOOLS miRNA RTqPCR primer/probe set, only common RT procedure required.
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Fast: RT reaction completed only within 15 mins.
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Accurate: Combination of an efficient reverse transcriptase and RNase inhibitor
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Efficient: Reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template
TOOLS Easy 2xProbe qPCR Mix
Cat#TTC-QE13
This optimized ready-to-load mix contains dNTP/dUTP mix, Mg2+, modified hot-start DNA polymerase, dUTP/UDG anticontamination system, specific ROX Reference Dye, and all components necessary to be directly used for a robust and low-template qPCR with high sensitivity, specificity, and reliability. The dUTP/UDG anticontamination system included into the mix eliminates the product contamination of qPCR reactions. TOOLS Easy 2×Probe qPCR Mix also contains ROX Reference Dye suitable for all qPCR instruments, and no adjustments are required for the dye concentration in different instruments.
Cat#TTC-QE13
This optimized ready-to-load mix contains dNTP/dUTP mix, Mg2+, modified hot-start DNA polymerase, dUTP/UDG anticontamination system, specific ROX Reference Dye, and all components necessary to be directly used for a robust and low-template qPCR with high sensitivity, specificity, and reliability. The dUTP/UDG anticontamination system included into the mix eliminates the product contamination of qPCR reactions. TOOLS Easy 2×Probe qPCR Mix also contains ROX Reference Dye suitable for all qPCR instruments, and no adjustments are required for the dye concentration in different instruments.
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Easy: All-in-one optimized master mix
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Specific: Premixed with dUTP/UDG anticontamination system
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High efficiency and stability
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ROX Reference Dye for fluorescent signal normalization and compatible with all qPCR instruments.
EasyPrep miRNA Extraction Kit
Cat#DPT-BE01
EasyPrep miRNA Extraction Kit utilizes a silica-based system to enrich and purify small RNA, including miRNA, small interfering RNA (siRNA), small nuclear RNA (snRNA), and total RNA from various sample sources (cell, animal tissue, plant tissue, serum, plasma), especially for small RNA of <200 nt. The lysis buffer in the kit is optimized to exert excellent lysis ability and isolation efficiency. High-quality isolated product without contamination of DNA and protein could be obtained within 1 hour, and directly used in Northern Blot, Dot Blot, Poly A screening, in vitro translation, RNase protection analysis, and also molecular cloning.
- Efficient: Completed within 1 hr
- Quality: silica-based system to yield high-purity small RNA product
- Flexible: Excellent lysis ability for various sample sources
Breast Cancer
- Rezaei, Zohreh, et al. "Involvement of the dysregulation of miR-23b-3p, miR-195-5p, miR-656-5p, and miR-340-5p in trastuzumab resistance of HER2-positive breast cancer cells and system biology approach to predict their targets involved in resistance." DNA and cell biology 38.2 (2019): 184-192.
- Balmeh, Negar, et al. "miR-195 down-regulation is a distinctive biomarker of HER2 positive state in breast cancer." Gene Reports 20 (2020): 100703.
- Yang, Rui, et al. "The circRNA circAGFG1 acts as a sponge of miR-195-5p to promote triple-negative breast cancer progression through regulating CCNE1 expression." Molecular cancer 18.1 (2019): 1-19.
Colorectal Cancer
- Sun, Min, et al. "Integrated analysis identifies microRNA-195 as a suppressor of Hippo-YAP pathway in colorectal cancer." Journal of hematology & oncology 10.1 (2017): 1-16.
- Falzone, Luca, et al. "Integrated analysis of colorectal cancer microRNA datasets: Identification of microRNAs associated with tumor development." Aging (Albany NY) 10.5 (2018): 1000.
- Lin, Xiaobin, et al. "miR-195-5p/NOTCH2-mediated EMT modulates IL-4 secretion in colorectal cancer to affect M2-like TAM polarization." Journal of hematology & oncology 12.1 (2019): 1-14.
- Feng, Chun, et al. "GDPD5, a target of miR-195-5p, is associated with metastasis and chemoresistance in colorectal cancer." Biomedicine & Pharmacotherapy 101 (2018): 945-952.
- Jin, Yinghu, et al. "Overcoming stemness and chemoresistance in colorectal cancer through miR-195-5p-modulated inhibition of notch signaling." International journal of biological macromolecules 117 (2018): 445-453.
- Jahromi, Zahra Forouzan, Arash Javeri, and Masoumeh Fakhr Taha. "Tumor suppressive effects of the pleiotropically acting miR-195 in colorectal cancer cells." EXCLI journal 18 (2019): 243.
Parkinson Disease
- Ding, Haixia, et al. "Identification of a panel of five serum miRNAs as a biomarker for Parkinson's disease." Parkinsonism & related disorders 22 (2016): 68-73.