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TOOLS Epstein-Barr Virus (EBV) miRNA Multiplex RT-qPCR Assay

User Guide MSDS
TOOLS EBV miRNA Multiplex RT-qPCR Assay includes designed and validated RT-qPCR primer/probe set targeting 44 respect EBV mature microRNAs (Table 1) that are suggested of crucial and potential roles in EBV related disease. This assay system utilizes a stem-looped primer to specifically extend the cDNA of target miRNA in reverse transcription (RT) and is an exclusively designed set for the probe-based qPCR assay to increase detection accuracy. Combined with TOOLSQuant II Fast RT kit and TOOLS Easy 2X probe qPCR mix, TOOLS EBV miRNA Multiplex RT-qPCR Assay System allows rapid, sensitive, and specific detection and quantification of 48 microRNAs in a total RNA sample respectively. TOOLS EBV miRNA Multiplex RT-qPCR Assay also includes the primer/probe sets for U6 and SNORD48 as the normalization controls and two exogenous miRNAs (cel-miR-39-3p, and cel-miR 238-3p) as spike-in controls
No. Size Price Qty
TTH-EBV 10 RT rxn / 20 qPCR rxn -
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Features
  • Well validated: targeting 44 respect EBV mature microRNAs that are suggested of crucial and potential roles in EBV related disease.  
  • High accuracy: Combined Reverse transcription with stem-looped primer with TagMan probe specially targeting mature miRNA.
  • Easy and fast
     Workflow

Sensitivity Tests

we using a serial dilution of synthetic cDNAs as templates in qPCR experiment to examined the #48 EBV primer/probe mix specificity. The synthetic cDNA templates (1X107 -1X101copies) were detected by qPCR reaction. The linear Range and sensitivity fell within the expected range.

Figure 1. High sensitivity with a range of synthetic cDNAs

 

Specificity Tests

We examined the #48 EBV primer Specificity using cross-hybridization of assay, using synthetic cDNAs as templates in qPCR experiment for assessing the corresponding qPCR primer/probe mix. Ct values (the threshold set as 0.2) are relevant to the Control Lot. The target signals fell within the expected range. The final assay conditions demonstrated no cross-hybridization of each primer. 

Figure 2. Broad linear range and high sensitivity