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mmu-miR-486a-3p

Datasheet User Guide
TOOL miRNA RT-qPCR primer/probe set is an integral part of TOOL miRNA RT-qPCR assay which is based on two-step RT-qPCR to especially target microRNAs. Each TOOLS miRNA RT-qPCR primer/probe set is packaged including two parts: one tube containing the unique designed stem-loop RT primer and the other containing a mix of the qPCR probe, forward, and reverse primers. 
No. Size Price Qty
TTH-M-486a-3p 50 rxns. $220.00
TTH-M-486a-3p 250 rxns $750.00
The price does not include shipping fee and tax. ORDER
Features
  • Comprehensive: quantitate only mature miRNAs, not precursors
  • Sensitive: sequence-specific primers
  • ​Efficient, simple, and scalable: two-step quantitative RT-PCR assay provides high-quality results in less than three hours

Assay information
miRBase Accession Number
MIMAT0017206
Chromosome Location
 chr8:23142629-23142649(+)
Mature miRNA Sequence:
CGGGGCAGCUCAGUACAGGAU
Species:
 Mus musculus
Assay System:
Probe-base qPCR assay
 

Design of TOOLS miRNA RT-qPCR assay system
Product name miRNA ACC
mmu-let-7a-5p MIMAT0000521
mmu-let-7b-5p MIMAT0000522
mmu-let-7c-5p MIMAT0000523
mmu-let-7d-3p MIMAT0000384
mmu-let-7d-5p MIMAT0000383
mmu-let-7f-5p MIMAT0000525
mmu-let-7g-5p MIMAT0000121
mmu-let-7i-3p MIMAT0004520
mmu-miR-1a-3p MIMAT0000123
mmu-miR-9-3p MIMAT0000143
mmu-miR-9-5p MIMAT0000142
mmu-miR-10a-3p MIMAT0004659
mmu-miR-10a-5p MIMAT0000648
mmu-miR-10b-5p MIMAT0000208
mmu-miR-15a-5p MIMAT0000526
mmu-miR-15b-3p MIMAT0004521
mmu-miR-15b-5p MIMAT0000124
mmu-miR-16-5p MIMAT0000527
mmu-miR-17-5p MIMAT0000649
mmu-miR-18a-5p MIMAT0000528
mmu-miR-19a-3p MIMAT0000651
mmu-miR-19b-3p MIMAT0000513
mmu-miR-20a-5p MIMAT0000529
mmu-miR-21a-5p MIMAT0000530
mmu-miR-22-3p MIMAT0000531
mmu-miR-22-5p MIMAT0004629
mmu-miR-23a-3p MIMAT0000532
mmu-miR-24-3p MIMAT0000219
mmu-miR-25-3p MIMAT0000652
mmu-miR-26a-1-3p MIMAT0017020
mmu-miR-26a-5p MIMAT0000533
mmu-miR-26b-5p MIMAT0000534
mmu-miR-27a-3p MIMAT0000537
mmu-miR-27b-3p MIMAT0000126
mmu-miR-296-3p MIMAT0004576
mmu-miR-29a-3p MIMAT0000535
mmu-miR-29b-3p MIMAT0000127
mmu-miR-30a-3p MIMAT0000129
mmu-miR-30a-5p MIMAT0000128
mmu-miR-30b-5p MIMAT0000130
mmu-miR-30c-5p MIMAT0000514
mmu-miR-30d-5p MIMAT0000515
mmu-miR-30e-3p MIMAT0000249
mmu-miR-30e-5p MIMAT0000248
mmu-miR-32-5p MIMAT0000654
mmu-miR-33-5p MIMAT0000667
mmu-miR-34a-5p MIMAT0000542
mmu-miR-92b-3p MIMAT0004899
Product name miRNA ACC
mmu-miR-93-5p MIMAT0000540
mmu-miR-98-5p MIMAT0000545
mmu-miR-99a-5p MIMAT0000131
mmu-miR-99b-5p MIMAT0000132
mmu-miR-100-5p MIMAT0000655
mmu-miR-101a-3p MIMAT0000133
mmu-miR-103-3pp MIMAT0000546
mmu-miR-106b-3p MIMAT0004582
mmu-miR-106b-5p MIMAT0000386
mmu-miR-107-3p MIMAT0000647
mu-miR-122-5p MIMAT0000246
mu-miR-125a-5p MIMAT0000135
mmu-miR-125b-5p MIMAT0000136
mmu-miR-126a-3p MIMAT0000138
mmu-miR-126a-5p MIMAT0000137
mmu-miR-127-3p MIMAT0000139
mmu-miR-128-3p MIMAT0000140
mmu-miR-130b-3p MIMAT0000387
mmu-miR-132-3p MIMAT0000144
mmu-miR-133a-3p MIMAT0000145
mmu-miR-133b-3p MIMAT0000769
mmu-miR-135a-1-3p MIMAT0004531
mmu-miR-135a-5p MIMAT0000147
mmu-miR-135b-5p MIMAT0000612
mmu-miR-140-3p MIMAT0000152
mmu-miR-141-3p MIMAT0000153
mmu-miR-142a-3p MIMAT0000155
mmu-miR-142a-5p MIMAT0000154
mmu-miR-143-3p MIMAT0000247
mmu-miR-144-3p MIMAT0000156
mmu-miR-145a-5p MIMAT0000157
mmu-miR-146a-5p MIMAT0000158
mmu-miR-148a-3p MIMAT0000516
mmu-miR-148a-5p MIMAT0004617
mmu-miR-148b-3p MIMAT0000580
mmu-miR-149-5p MIMAT0000159
mmu-miR-150-5p MIMAT0000160
mmu-miR-181a-1-3p MIMAT0000660
mmu-miR-181a-5p MIMAT0000210
mmu-miR-183-5p MIMAT0000212
mmu-miR-184-3p MIMAT0000213
mmu-miR-185-5p MIMAT0000214
mmu-miR-186-5p MIMAT0000215
mmu-miR-187-3p MIMAT0000216
mmu-miR-191-5p MIMAT0000221
mmu-miR-192-5p MIMAT0000517
mmu-miR-193b-5p MIMAT0017271
mmu-miR-194-5p MIMAT0000224
Product name miRNA ACC
mmu-miR-195a-5p MIMAT0000225
mmu-miR-196a-5p MIMAT0000518
mmu-miR-196b-5p MIMAT0001081
mmu-miR-199a-3p MIMAT0000230
mmu-miR-200a-3p MIMAT0000519
mmu-miR-200c-3p MIMAT0000657
mmu-miR-203-3p MIMAT0000236
mmu-miR-204-3p MIMAT0017002
mmu-miR-204-5p MIMAT0000237
mmu-miR-205-5p MIMAT0000238
mmu-miR-208a-3p MIMAT0000520
mmu-miR-208b-3p MIMAT0004939
mmu-miR-210-3p MIMAT0000658
mmu-miR-214-3p MIMAT0000661
mmu-miR-218-5p MIMAT0000663
mmu-miR-221-3p MIMAT0000669
mmu-miR-223-3p MIMAT0000665
mmu-miR-320-3p MIMAT0000666
mmu-miR-328-3p MIMAT0000565
mmu-miR-335-3p MIMAT0004704
mmu-miR-335-5p MIMAT0000766
mmu-miR-338-3p MIMAT0000582
mmu-miR-340-5p MIMAT0004651
mmu-miR-342-3p MIMAT0000590
mmu-miR-365-1-5p MIMAT0017077
mmu-miR-365-3p MIMAT0000711
mmu-miR-367-3p MIMAT0003181
mmu-miR-374b-5p MIMAT0003727
mmu-miR-375-3p MIMAT0000739
mmu-miR-378a-5p MIMAT0000742
mmu-miR-409-3p MIMAT0001090
mmu-miR-421-3p MIMAT0004869
mmu-miR-423-3p MIMAT0003454
mmu-miR-423-5p MIMAT0004825
mmu-miR-425-5p MIMAT0004750
mmu-miR-450a-5p MIMAT0001546
mmu-miR-451a MIMAT0001632
mmu-miR-455-5p MIMAT0003485
mmu-miR-486a-3p MIMAT0017206
mmu-miR-494-3p MIMAT0003182
mmu-miR-499-5p MIMAT0003482
mmu-miR-532-5p MIMAT0002889
mmu-miR-574-3p MIMAT0004894
mmu-miR-615-3p MIMAT0003783
mmu-miR-671-5p MIMAT0003731
mmu-miR-744-5p MIMAT0004187
mmu-miR-877-5p MIMAT0004861
mmu-miR-1247-5p MIMAT0014800
TOOLS miRNA RT Kit
Cat#TTH-mi50 / TTH-mi250

TOOLS miRNA RT Kit provides the perfect first step of TOOLS miRNA RT-qPCR assay. This Kit adopts an innovative RT enzyme and buffer system, which enables the RT reaction completed within only 15 mins, and provides reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template.
  • Easy: Compatible with TOOLS miRNA RTqPCR primer/probe set, only common RT procedure required. 
  • Fast:  RT reaction completed only within 15 mins.
  • Accurate: Combination of an efficient reverse transcriptase and RNase inhibitor
  • Efficient: Reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template
TOOLS Easy 2xProbe qPCR Mix
Cat#TTC-QE13

This optimized ready-to-load mix contains dNTP/dUTP mix, Mg2+, modified hot-start DNA polymerase, dUTP/UDG anticontamination system, specific ROX Reference Dye, and all components necessary to be directly used for a robust and low-template qPCR with high sensitivity, specificity, and reliability. The dUTP/UDG anticontamination system included into the mix eliminates the product contamination of qPCR reactions.  TOOLS Easy 2×Probe qPCR Mix also contains ROX Reference Dye suitable for all qPCR instruments, and no adjustments are required for the dye concentration in different instruments.
  • Easy: All-in-one optimized master mix
  • Specific: Premixed with dUTP/UDG anticontamination system
  • High efficiency and stability
  • ROX Reference Dye for fluorescent signal normalization and compatible with all qPCR instruments.
EasyPrep miRNA Extraction Kit
Cat#DPT-BE01

EasyPrep miRNA Extraction Kit utilizes a silica-based system to enrich and purify small RNA, including miRNA, small interfering RNA (siRNA), small nuclear RNA (snRNA), and total RNA from various sample sources (cell, animal tissue, plant tissue, serum, plasma), especially for small RNA of <200 nt. The lysis buffer in the kit is optimized to exert excellent lysis ability and isolation efficiency. High-quality isolated product without contamination of DNA and protein could be obtained within 1 hour, and directly used in Northern Blot, Dot Blot, Poly A screening, in vitro translation, RNase protection analysis, and also molecular cloning.
  • Efficient: Completed within 1 hr
  • Quality: silica-based system to yield high-purity small RNA product
  • Flexible: Excellent lysis ability for various sample sources